CRISPR-TB Point-of-Care Blood Assay
Rapid, Accessible, and Ultra-Sensitive TB Detection at the Point of Care
IntelliGenome introduces its first point-of-care (POC) molecular diagnostic solution, the CRISPR-TB POC Blood Assay*, designed to bring ultra-sensitive tuberculosis (TB) detection closer to patients. This single-test solution detects Mycobacterium tuberculosis (Mtb) DNA from nucleic acids in plasma or serum, enabling the accurate, rapid, and affordable diagnosis of both pulmonary (PTB) and extra-pulmonary tuberculosis (EPTB) across all patient groups.
Self-Contained & Simplified Testing
The CRISPR-TB POC Blood Assay is a self-contained diagnostic system with minimal sample collection and processing requirements, making it ideal for decentralized testing in clinics, rural areas, and resource-limited settings.
Lab in Tube (LIT): Next-Generation POC TB Testing
Building on its pioneering CRISPR diagnostic technology, IntelliGenome has developed Lab in Tube (LIT)*, an advanced point-of-care molecular diagnostic platform designed for portability, ease of use, and accessibility.
Key Features of Lab in Tube (LIT)
- All-in-One Testing Device – Integrates all necessary reagents to isolate and detect Mtb cfDNA in a single, compact device.
- Portable & Scalable – Designed for use in resource-constrained regions, making TB testing accessible for all patient groups, regardless of age or health conditions.
- Fluorescent Signal Detection – Works with a portable, reusable fluorescent signal reader for accurate real-time analysis.
How It Works: Three-Stage Testing System
- DNA Amplification Module: Contains high-fidelity DNA polymerase, PCR buffer with stabilization proteins, dNTPs, and primer pairs targeting TB IS-6110 sequences for nucleic acid amplification.
- CRISPR Detection Module: Incorporates a Cas enzyme reaction with a specially formulated reaction buffer, fluorescent probes, gRNA, and disposable paper strips for CRISPR-based target detection.
- TB Identification Module: Features paper strips for precise TB-IS6110 detection, a DNA sequence unique to Mycobacterium tuberculosis. These strips provide clear visual confirmation of test results.
Flexible & Cost-Effective Deployment
- Disposable Test Kits – LIT components are single-use and designed for rapid, on-site TB testing.
- Portable Fluorescent Readers– Sold separately for long-term, cost-efficient use across multiple tests.